Lactzyme Co., Ltd.

About Lactzyme

Lactic acid bacteria producing substance and lactic acid bacteria

In recent years, with the progress of science and technology, the close relationship with health, aging, and intestinal environment has become clear. Until then, what was happening in the intestine was unknown as chaos, but now that instruments for genetic analysis have been sold at a low price, it has become possible to see the work of intestinal flora one after another, Research on intestinal flora (Enterobacteriaceae clustered like a flower garden) at universities, research institutes, and companies is accelerating.

These studies have revealed that intestinal flora affects the life support mechanism such as immune function, as well as intestinal flora works to the brain, and the intestine is called the "second brain" It came to be. However, no matter how much research has progressed, there is still no clear answer to what the gut should be for health.

On the other hand, in Japan, which is a longevity country, sales battles for lactic acid bacteria and dietary fiber are taking place in the intestinal flora.
While a lactic acid bacteria beverage manufacturer advertises on TV commercials that "aging is from the intestines" and "take care for the intestines", just taking these lactic acid bacteria products and supplements will wonder if they will get away from the disease and become healthy I think there are many people.
The close relationship between aging and intestinal flora can make an image, but there is no convincing explanation of what to do with intestinal flora.

The method of using intestinal flora proposed by us is completely different from the above method of drinking lactic acid bacteria directly. Our method is a new concept of using Bio-Products, which jumps over intestinal flora, which is a collection of enteric bacteria, directly approaches the immune system that supports our health, and uses the entire system.

Bio-Products = Lactic acid bacteria-producing Bio-Products = Lactzyme(LZ)

Bio-Products refers to the components contained in the liquid produced and released during the growth of lactic acid bacteria.
In the first place, the general public has almost no chance to see the components produced by lactic acid bacteria, so I think that the image does not come out, but among researchers, it is called "Bio-product" or "metabolite". Bio-Products is often compared to silkworms and silk. A cocoon eats mulberry leaves to make silk thread, but of course, the mulberry leaves and the silkworm body is not made of silk.
The silk thread produced by silkworms using mulberry leaves as a nutritional source, can be compared to how lactic acid bacteria produce Bio-Products using the food we eat as a nutritional source.

Living thing
to use
Silkworm Intestinal flora
(Lactic acid bacteria・bifidobacteria)
蚕 乳酸菌・ビフィズス菌
Size 2mm〜80mm 0.0005mm〜0.01mm
Comfortable temperature 25℃ 36℃
Nutrition Mulberry leaves Sugars such as glucose
Manufacturing process 蚕の繭
cocoon
ヨーグルト
Fermented milk (soy milk)
Ingredients used
as products
生糸
Raw silk
サナギ
pupa
Bio-Products
Bio-Products
菌体、死菌体
Viable bacteria,
Dead bacteria
Products made 絹織物
Silk fabric
佃煮
Tsukudani, feed, etc.
回輝源
"Kai-ki-gen" etc.
ヨーグルト
Yogurt
Product ingredients Silk100% pupa 100% extract produced
by the fungus
Viable bacteria,
Dead bacteria,
Trace amounts of Bio-Products
Product features Not suitable
for mass production
Hardly circulated Does not contain bacterial components.
It takes time and effort.
Not suitable for mass production.
Suitable for mass production
Available quantity Few Few Few numerous
Price Expensive Hardly circulated Expensive as food Inexpensive

While research on intestinal flora and immune function has progressed, the idea of ​​increasing profitable bacillus (useful bacteria) by sending ingredients into the intestine as a food that has a function to increase profitable bacillus in the intestine was born.
As a result, foods with various functions will be sold divided into genres such as Prebiotics (oligosaccharides, dietary fiber, etc.) and Prebiotics (Yogurt containing live good bacteria such as lactic acid bacteria beverages and bifidobacteria). Recently, synbiotics that combine Probiotics and Prebiotics also appeared, and a boom with products related to lactic acid bacteria has arrived.
However, the idea of ​​increasing the number of good bacteria currently in the intestines has the following questions and problems.

  • ● Drinking lactic acid bacteria does not always work in the intestines
  • ● It is doubtful if it is meaningful to increase your own good bacteria that age with aging
  • ● You cannot supplement your own intestinal flora with other people's bacteria
  • ● The fact that stool was promoted after drinking lactic acid bacteria may be moving to excrete foreign bodies
  • ● The intestines and immune system are likely to require different things

Therefore, a new genre product was born to eliminate these problems.
Focusing on the function that intestinal bacteria control our health, it is not the purpose of supplementing the number of existing "profitable bacillus", it works directly on the immune system near the intestinal tract and seems to work like "profitable bacillus". It is a product called “Biogenics”.

The representative player of Biogenics is Lactzyme (Kai-ki-gen) that we provide. Lactzyme (Kai-ki-gen) is a product that is produced by reproducing the components released by bacteria when intestinal flora called "Profitable bacillus" such as lactic acid bacteria grows in the intestine and purified as an extract.

Lactic acid bacteria producing substance and lactic acid bacteria

Our Intestinal wall has a mechanism that recognizes a creature called intestinal flora and sends a signal to the immune system according to the state.
On the other hand, it is also known that components such as Biological-Products, called products released by intestinal bacteria, are absorbed directly from the intestinal wall, causing the immune system to move directly. Even if you try to send a signal from the Intestinal wall after drinking Lactic acid bacteria, there is a high possibility that other bacteria will be excluded, but if it is a metabolite (production extract) of lactic acid bacteria, itself is not the bacteria, so it can expect to be absorbed without being eliminated.

Bio-Products = Lactic acid bacteria-producing Bio-Products = Lactzyme(LZ)

What the intestine wants

The first thing that comes to mind is digestible food. Foods that are said to be stomach-friendly, such as “Rice gruel”, are one of the things that are appreciated for intestinal digestion and absorption.
The intestine is an organ that digests and absorbs food, but on the other hand, it is also a source of resistance and an immune organ. Let's change the viewpoint and think about what is beneficial for the intestines as an immune organ.

The demands of the intestines as an immune organ change a little. If the number of good bacteria increases, the secretion of good bacteria will increase, and it is thought that the ingredients that increase the immunity contained in it will also increase.
In general, foods that increase good bacteria and increase immunity tend to be difficult to digest and absorb. Because many prebiotics (dietary fiber, etc.) that are said to increase good bacteria are difficult to be digested by digestive enzymes secreted in the intestines, they are difficult to absorb as a nutrient source.

This feature is used for diet, However, because it is broken down by intestinal flora, there are many things that are said to be poorly digested.
Germ, bamboo shoots, corn, etc. are poorly digested, but can also be said to be intestinal flora's favorite food. If you think so, it's hard to say that it's bad for your body just because it's indigestible food.
Besides this, good bacteria prefer to digest oligosaccharides and high-quality proteins. Oligosaccharides are “Indigestible sugars” for humans.
So, for the immune system, is it the most appreciated thing to increase good bacteria with these prebiotics?

Inconvenient truth

If it doesn't make much sense to replenish good bacteria from outside, is it effective to add prebiotics to the good bacteria in the intestine to promote their growth and make them good?
The answer depends on the age of the target person.

If we conclude the conclusion earlier, “The good bacteria that have deteriorated due to aging are difficult to grow no matter how nutrients are given”.
This can be seen at a glance by collecting good bacteria from the feces of old people, culturing them, and comparing them with the young good bacteria given the same nutrients. In the young intestines, good bacteria are dominant, metabolites of good bacteria are secreted abundantly, and always stimulate immune system cells through the intestinal wall.
However, as the number of good bacteria decreases with age and metabolites decrease, the immunity also decreases in response. Looking at the state of the intestinal flora of the old man, not only the number of good bacteria is small, but also in terms of "Activity", that is, whether the bacteria are fine, the bacteria are also aging according to their age.
If the number of bad bacteria increases, metabolites of bad bacteria may affect the growth and activity of good bacteria.
Please look at the graph of age change of intestinal flora below again.
Overlaid on the graph showing the decrease in the number of good bacteria created by Mitsuoka (Professor Emeritus, The University of Tokyo), We tried to express the decrease in immunity as the color of the bar graph that changes from blue to red.

It is often said that “Aging is from the intestines” and “Let's take care of the intestines”. Sure, aging and intestinal flora are closely related, but no description that gives a clear answer on how to take care of them is found in the materials of lactic acid bacteria product sales companies.
It is excreted in the end, no matter how good good bacteria are supplemented. No matter how much you eat probiotics and increase intestinal flora aged, you can't expect to secrete high-quality metabolites. Even if you know it, you can't say it clearly because if you say these, the product will not sell.
For a major lactic acid bacteria product sales company that leads the market for lactic acid bacteria foods, this may be exactly 『Inconvenient truth(※)』.

※『Inconvenient truth』:Created by former US Vice President Al Gore, Documentary movie depicting the global crisis due to global warming

Bio-Products = Lactic acid bacteria-producing Bio-Products = Lactzyme(LZ)

Yogurt is famous fermented foods found anywhere in the world. Soy beans are very effective likewise as a lifestyle-related disease prevention food.  In the later stage of fermented soy beans development by lactic acid bacterium becomes clear. Lz is a natural substances that is released by intestinal bacteria which has similar effects to antibiotic In other words Lactbacillus Products LZ is the same secreted products of intestinal bacteria in our intestine.

1.Adiponectine and LZ

■ Insulin and Diabetes
Insulin is a hormone made by the pancreas that enables cells to take in glucose from the blood and use it for energy. Failure to produce insulin, or of insulin to act properly, or both, leads to raised glucose levels in the blood (hyperglycaemia). This is associated with long-term damage to the body and failure of various organs and tissues. Diabetes is a chronic disease that arises when the pancreas does not produce enough insulin(type-1), or when the body cannot effectively use the insulin it produces(type-2).

■ A fat cell and insulin resistance
The greatest cause of type-2 diabetes as a lifestyle related disease is corpulence, and insulin resistance is brought as a result of TNF-α, registine hormone, which is secreted by enlarged fat cells, The effects of insulin decrease is called insulin resistance.

■ Adiponectin production 2.2 times
As for adiponectine hormone in the blood, also it is known as" Anti-aging hormone".  It helps a work of insulin and reduce the amount of grease in the fat calls. A fall of adiponectine accepted as one of the causes of metabolic syndrome. Our study team found that the level of adiponectine hormone increased by 2.2 times the normal level. When LZ was absorbed by fat cells. We found that only LZ had a effect of increasing adiponectine such a significant effect, We found that LZ promoted adiponectine in 1st generation organ fat cells obtained from trial rats. In other words LZ is the ingredient works on a fat cell and raises adiponectine production ability. Our team announced this study contents for the first time in the world in a Japan Society of Nutrition and Food science done in May, 2007.

The functionality of Bio-Products (Lactzyme) has important implications not only for suppressing adipocyte proliferation but also for preventing lifestyle-related diseases that are caused in a chain. There is no other material like Bio-Products (Lactzyme) that suppresses adipocyte proliferation and increases Adiponectin production.



  • ◉ Anti-diabetic / Anti-arteriosclerotic / Anti-inflammatory / Anti-obesity
  • ◉ Immunostimulation / Anti-allergy / Anti-virus
  • ◉ Delay aging

2.Suppresses blood sugar growth

Diabetes currently accounts for over 13 million patients (2017), including the reserve army, in Japan. Therefore, we examined the effects of Bio-Products (Lactzyme) on diabetes. There is an animal called KK-Ay mouse that develops severe diabetes 7 to 8 weeks after birth by genetic engineering. This animal is also used as evidence when pharmaceutical manufacturers apply to the Ministry of Health, Labor and Welfare for treatment of diabetes. The functionality of how Bio-Products (Lactzyme) suppresses blood glucose levels in diabetic mice is obvious as shown in the graph. And simultaneous experiments with skeletal muscle cells showed an increase in the number of Glut4 molecules that act as a bridge to take up glucose (sugar) into the cells. Cell experiments confirmed that taking Bio-Products (Lactzyme) may increase glucose uptake and lower blood glucose levels in skeletal muscle cells.

3.Macrophages and insulin resistance

In general, Macrophage is said to be activated by receiving cytokines produced by T cells. Certain types of Cytokine promote monocyte maturation, proliferate Macrophage and activate phagocytosis.
It is generally known that there are two types of macrophage activation as described above.
It is the classical activation of M1 by a cell component called “LPS”, which is often used in immune system experiments, and the selective activation of M2 by cytokines such as IL-4 and IL-13.
In the above analysis of M1 / ​​M2 expression, visceral fat macrophage stimulation by LPS was performed as a control, and a drastic decrease in M2 macrophage was observed by LPS stimulation.
However, it is considered that there are three or more intermediate activation states of [Classical Activation Macrophage], [Wound Healing Macrophage], and [Controlled Macrophage] in the more recent classification, these different phenotype macrophages are thought to be expressed by LZ stimulation.
In addition to this, Macrophage distributed throughout the body has various functions, Microglia cells in the brain also have various functions, Histospheres (Langerhans cells) in the skin, Kupffer cells in the liver, and dust cells (alveolar macrophages) in the alveoli are also macrophages.
If the relationship between LZ and M1 / ​​M2 Macrophage becomes clear, We would like to examine the action of these cells as a mechanism of the effect of LZ in clinical practice that has been reported by customers (the reason why it is cured by LZ).

In general, fat accumulation in fat cells is known to cause insulin resistance, However In this scene, Macrophage plays an important role in the adipose tissue, and it has been revealed from the numerous experiments we have conducted to confirm the production of adiponectin.
Macrophage is a representative player of phagocytic cells that processes cancer cells and is often regarded as a classic immune cell, However Recently, research on obesity has progressed, and it has been widely announced at the academic level that the types of macrophages present in obese and non-obese adipose tissues are different.
Two types of phenotypes in Macrophage in adipose tissue are divided into “M1 Macrophage” that produces inflammatory cytokines and “M2 Macrophage” that produces anti-inflammatory cytokines.
M1 Macrophage is widely known to promote insulin resistance and is closely related to insulin resistance due to excessive accumulation of adipocytes.
On the other hand, the “non-inflammatory” of M2 Macrophage works in opposition to the M1 type, and it can be said that it is actually a “regenerative” Macrophage.
In other words, Inflammatory macrophage (M1) increases in obese adipose tissue, and Non-inflammatory macophage (M2)] increases in non-obese adipose tissue. That is it has become clear that inflammatory macrophage (M1), especially in obese adipose tissue, is greatly involved in the development of lifestyle-related diseases.



M1 inflammatory macrophage and M2 non-inflammatory macrophage


There are many things in our bodies that have conflicting functions. For example, the nerve tissue complements each other, such as the exchange nerve and the parasympathetic nerve, so that they are balanced.
To correspond to the above M1 type and M2 type, there are two types of Macrophage present in adipocytes: Adhesive (ADM) and Floating (FLM), We have confirmed by our experiments until the floating macrophage increases due to the action of LZ.
As a result of joint research with Primary Cell Co., Ltd. in 2007, we announced that it promotes adiponectin production from rat primary visceral fat cells (VAC), but at the same time, when LZ is added to VAC, it is announced that “a lot of floating Macrophage will appear”.
However, Floating Macrophage in fat cells is not necessarily M2 type.
In response, After confirming these points in experiments using an additive-free VAC culture system, in November 2008, at the Biochemical Society held in Kobe, As a result of joint research with Primary Cell Co., Ltd., we presented research on two phenotypes.
The general content of the announcement is,

● In the excessively accumulated fat cells, M1-type Macrophage is dominant.
● On the other hand, in the state of not being obese, M2 type Macrophage is dominant.
● Adhesive Macrophage (ADM) is M1 dominant.
● Floating Macrophage (FLM) is M2 dominant.

In other words,『Floating Macrophage found that there are many M2 type』.

4.Suppression of adipocyte proliferation

Although it can be imagined that Bio-Products (Lactzyme) directly acts on human immune cells through the intestinal wall, Bio-Products (Lactzyme) may have high possibility of affecting the whole body glucose metabolism and fat metabolism. In other words, our research also revealed that there is a possibility of improving metabolic syndrome (visceral fat obesity). In experiments where Bio-Products (Lactzyme) is added to adipocytes, It has been confirmed that the effect of inhibiting fat growth far exceeds that of fermented tea, which is marketed as having an effect on visceral fat.

Enteric bacteria ▶︎ The fat cells 4 days after feeding the normal nutrients to the experimental fat cells are quite large.
Enteric bacteria ▶︎ Photograph of the above cells cultured with fermented tea added. The number of proliferated adipocytes looks slightly smaller.
Enteric bacteria ▶︎ Cultured using Bio-Products (Lactzyme) instead of fermented tea. Adipocytes are no larger than a certain size.

5.Future possibility of LZ

According to a certain experiment with a mouse, lipid accumulation in a macrophage is restrained by and understands that arteriosclerosis is restrained by adiponectine. It is thought adiponectine has arteriosclerosis restraint action as well as insulin resistance improvement action. Increasing adiponectine will result in a higher probability that good health will be restored. We found that Biological products LZ to be an amazing product as its effect is very rare and difficult to replace.